Knockdown of the Placental Growth Factor Gene Inhibits Laser Induced Choroidal Neovascularization in a Murine Model
نویسندگان
چکیده
PURPOSE To evaluate the effect of placental growth factor (PlGF) gene knockdown in a murine model of laser-induced choroidal neovascularization. METHODS Choroidal neovascularization was induced in the left eyes of 11 mice by infrared laser. Small interfering RNA (siRNA, 20 picomoles/10 μl) corresponding to PlGF mRNA was administered intravitreally by Hamilton syringe in all subjects. One month later, fluorescein angiography and histolologic examination were performed. RESULTS No leakage was apparent in the 11 eyes treated with siRNA cognate to PlGF. The results of histological evaluation were consistent with angiographic findings showing absence of choroidal neovascularization. CONCLUSION Knockdown of the PlGF gene can inhibit the growth of laser-induced choroidal neovascularization in mice.
منابع مشابه
Placental growth factor, a member of the VEGF family, contributes to the development of choroidal neovascularization.
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